Surface modification to reduce nonspecific binding of quantum dots in live cell assays.

Bentzen EL, Tomlinson ID, Mason J, Gresch P, Warnement MR, Wright D, Sanders-Bush E, Blakely R, Rosenthal SJ
Bioconjug Chem. 2005 16 (6): 1488-94

PMID: 16287246 · DOI:10.1021/bc0502006

Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units.

MeSH Terms (9)

Acrylic Resins Animals Binding Sites Cell Line Cytological Techniques Fluorescent Dyes Polyethylene Glycols Quantum Dots Surface Properties

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