Regulation of telomere length by an N-terminal region of the yeast telomerase reverse transcriptase.

Ji H, Platts MH, Dharamsi LM, Friedman KL
Mol Cell Biol. 2005 25 (20): 9103-14

PMID: 16199886 · PMCID: PMC1265764 · DOI:10.1128/MCB.25.20.9103-9114.2005

Telomerase is a reverse transcriptase that maintains chromosome integrity through synthesis of repetitive telomeric sequences on the ends of eukaryotic chromosomes. In the yeast Saccharomyces cerevisiae, telomere length homeostasis is achieved through negative regulation of telomerase access to the chromosome terminus by telomere-bound Rap1 protein and its binding partners, Rif1p and Rif2p, and positive regulation by factors such as Ku70/80, Tel1p, and Cdc13p. Here we report the identification of mutations within an N-terminal region (region I) of the yeast telomerase catalytic subunit (Est2p) that cause telomere lengthening without altering measurable catalytic properties of the enzyme in vitro. These telomerase mutations affect telomere length through a Ku-independent mechanism and do not alter chromosome end structure. While Tel1p is required for expression of the telomere-lengthening phenotype, Rif1p and Rif2p are not, suggesting that telomere overextension is independent of Rap1p. Taken together, these data suggest that specific amino acids within region I of the catalytic subunit of yeast telomerase play a previously unanticipated role in the response to Tel1p regulation at the telomere.

MeSH Terms (17)

Alleles Base Sequence Carrier Proteins Catalytic Domain DNA, Fungal DNA-Binding Proteins Fungal Proteins Genes, Fungal Intracellular Signaling Peptides and Proteins Mutation Protein-Serine-Threonine Kinases Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Telomerase Telomere Telomere-Binding Proteins

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