Effects of age and caloric restriction on lipid peroxidation: measurement of oxidative stress by F2-isoprostane levels.

Ward WF, Qi W, Van Remmen H, Zackert WE, Roberts LJ, Richardson A
J Gerontol A Biol Sci Med Sci. 2005 60 (7): 847-51

PMID: 16079206 · DOI:10.1093/gerona/60.7.847

The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.

MeSH Terms (20)

8-Hydroxy-2'-Deoxyguanosine Age Factors Aging Animal Nutritional Physiological Phenomena Animals Biomarkers Caloric Restriction Chromatography, High Pressure Liquid Chromatography, Thin Layer Deoxyguanosine DNA F2-Isoprostanes Gas Chromatography-Mass Spectrometry Kidney Lipid Peroxidation Liver Male Oxidative Stress Rats Rats, Inbred F344

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