Effects of age and caloric restriction on lipid peroxidation: measurement of oxidative stress by F2-isoprostane levels.

Ward WF, Qi W, Van Remmen H, Zackert WE, Roberts LJ, Richardson A
J Gerontol A Biol Sci Med Sci. 2005 60 (7): 847-51

PMID: 16079206 · DOI:10.1093/gerona/60.7.847

The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.

MeSH Terms (19)

Age Factors Aging Animal Nutritional Physiological Phenomena Animals Biomarkers Caloric Restriction Chromatography, High Pressure Liquid Chromatography, Thin Layer Deoxyguanosine DNA F2-Isoprostanes Gas Chromatography-Mass Spectrometry Kidney Lipid Peroxidation Liver Male Oxidative Stress Rats Rats, Inbred F344

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