Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry.

Norris JL, Porter NA, Caprioli RM
Anal Chem. 2005 77 (15): 5036-40

PMID: 16053319 · DOI:10.1021/ac050460g

This study reports the synthesis of the first functional cleavable detergent designed specifically for applications in mass spectrometry. Upon cleavage, two inert compounds and the MALDI matrix are formed, eliminating sources of potential interference originating from traditional cleavable detergents. Analysis of peptides demonstrates that MALDI matrix generated in situ results in MALDI spectra equivalent to those prepared using established protocols. Analysis of the membrane protein diacylglycerol kinase was accomplished using the combination detergent/MALDI matrix. Applications of the functional cleavable detergents to the profiling of whole cell lysates results in increased signal-to-noise ratios of many ions and the detection of additional proteins previously not observed.

MeSH Terms (9)

Acrylates Cell Line Detergents Escherichia coli Membrane Proteins Molecular Structure Peptides, Cyclic Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature

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