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Differential effects of amphiregulin and TGF-alpha on the morphology of MDCK cells.

Chung E, Graves-Deal R, Franklin JL, Coffey RJ
Exp Cell Res. 2005 309 (1): 149-60

PMID: 15979068 · DOI:10.1016/j.yexcr.2005.05.012

Although both amphiregulin and TGF-alpha are known to exert their effects through the EGF receptor, we find that concentrations of recombinant human amphiregulin and TGF-alpha that are equipotent in EGF receptor activation and mitogenesis exhibit markedly different effects on MDCK cell morphology. Amphiregulin induces a spindle-like morphology that is associated with a redistribution of E-cadherin from a Triton-insoluble to Triton-soluble pool. TGF-alpha does not affect epithelial morphology nor does it affect the distribution of the Triton-soluble or -insoluble pool of E-cadherin. The effects of amphiregulin on E-cadherin are associated with actin rearrangement. The morphological and biochemical effects of amphiregulin are prevented by EGF receptor blockade but require Src-family kinase activity and MAPK signaling. These results identify an action of amphiregulin that is distinct from TGF-alpha that may contribute to amphiregulin's participation in the pathogenesis of inflammatory disorders like psoriasis and cancer.

MeSH Terms (14)

Amphiregulin Animals Cell Differentiation Cell Line Cell Shape Dogs EGF Family of Proteins ErbB Receptors Glycoproteins Intercellular Signaling Peptides and Proteins MAP Kinase Signaling System Phosphorylation Recombinant Proteins Transforming Growth Factor alpha

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