pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.

Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT
Proc Natl Acad Sci U S A. 1992 89 (11): 5192-6

PMID: 1594631 · PMCID: PMC49256 · DOI:10.1073/pnas.89.11.5192

Expression of the Rous sarcoma virus-encoded oncoprotein, pp60v-src, subverts the normal regulation of cell growth, which results in oncogenic transformation. This process requires the intrinsic protein-tyrosine kinase activity of pp60v-src and is associated with an increase in tyrosine phosphorylation of a number of cellular proteins, candidate substrates for pp60v-src. We report here the isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotyrosine in pp60v-src-transformed chicken embryo cells. This cDNA encodes a cytoplasmic protein-tyrosine kinase which, based upon its predicted amino acid sequence and structure, is the prototype for an additional family of protein-tyrosine kinases. Immunofluorescence localization experiments show that pp125 is localized to focal adhesions; hence, we suggest the name focal adhesion kinase.

MeSH Terms (21)

Amino Acid Sequence Animals Base Sequence Cell Adhesion Cell Adhesion Molecules Cell Transformation, Viral Chick Embryo Cloning, Molecular Cytoskeletal Proteins DNA Fluorescent Antibody Technique Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Microfilament Proteins Molecular Sequence Data Molecular Weight Phosphoproteins Protein-Tyrosine Kinases Restriction Mapping Sequence Alignment Tensins

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