Solution NMR spectroscopy of the human vasopressin V2 receptor, a G protein-coupled receptor.

Tian C, Breyer RM, Kim HJ, Karra MD, Friedman DB, Karpay A, Sanders CR
J Am Chem Soc. 2005 127 (22): 8010-1

PMID: 15926814 · DOI:10.1021/ja051161b

The seven-transmembrane-spanning G protein-coupled receptor (GPCR) superfamily plays many important roles in basic biology, human health, and human disease. Here, well-resolved solution NMR spectra are presented for a human GPCR, the vasopressin V2 receptor in detergent micelles. The quality of the NMR spectra indicates that backbone resonance assignments for a majority of resonances are feasible. The key to obtaining high quality spectra appears to be the coupling of methods for expressing the receptor into membranes rather than into inclusion bodies, with use of a biochemically mild lysolipid detergent for membrane extraction, protein purification, and NMR sample preparation.

MeSH Terms (8)

Escherichia coli Humans Nuclear Magnetic Resonance, Biomolecular Protein Structure, Tertiary Receptors, G-Protein-Coupled Receptors, Vasopressin Recombinant Proteins Solutions

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