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Identification of an H-2D(b)-restricted CD8+ cytotoxic T lymphocyte epitope in the matrix protein of respiratory syncytial virus.

Rutigliano JA, Rock MT, Johnson AK, Crowe JE, Graham BS
Virology. 2005 337 (2): 335-43

PMID: 15916793 · DOI:10.1016/j.virol.2005.04.032

Cytotoxic T lymphocytes (CTL) play a significant role in the clearance of respiratory syncytial virus (RSV) infection in humans and mice. Identification of class I MHC-restricted CTL epitopes is critical in elucidating mechanisms of CTL responses against viral infections. However, only four H-2d-restricted epitopes have been reported in mice. Because of the diversity of transgenic and knockout mice available to study immune responses, new epitopes in additional strains of mice must be identified. We therefore attempted to discover novel CTL epitopes in C57Bl/6 mice. Our efforts revealed a new H-2D(b)-restricted CTL epitope from the RSV M protein, corresponding to aa 187-195 (NAITNAKII). Also, M187-195-specific CTLs were activated with kinetics similar to the immunodominant BALB/c epitope, M2 82-90. This is the first RSV-specific CTL epitope described in a strain of mice other than BALB/c. Furthermore, identification of this H-2b-restricted CTL epitope provides access to genetically modified H-2b mice for more detailed studies of CTL mechanisms in RSV infection.

MeSH Terms (19)

Amino Acid Sequence Animals Cell Line, Tumor Cytokines Disease Models, Animal Epitopes Female H-2 Antigens Histocompatibility Antigen H-2D Humans Major Histocompatibility Complex Mice Mice, Inbred BALB C Molecular Sequence Data Peptide Fragments Respiratory Syncytial Viruses Respiratory Syncytial Virus Infections T-Lymphocytes, Cytotoxic Viral Matrix Proteins

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