Genomic Comparison of cag pathogenicity island (PAI)-positive and -negative Helicobacter pylori strains: identification of novel markers for cag PAI-positive strains.

Terry CE, McGinnis LM, Madigan KC, Cao P, Cover TL, Liechti GW, Peek RM, Forsyth MH
Infect Immun. 2005 73 (6): 3794-8

PMID: 15908415 · PMCID: PMC1111835 · DOI:10.1128/IAI.73.6.3794-3798.2005

In an analysis of Helicobacter pylori genomic DNA by macroarray methodology, genomic DNA from a panel of cag pathogenicity island (PAI)-negative H. pylori clinical isolates failed to hybridize with 27 genes located outside the cag PAI in a cag PAI-positive reference strain. PCR analyses confirmed that HP0217 (encoding a lipopolysaccharide biosynthetic protein) and HP1079 (encoding a protein of unknown function) were present significantly more frequently in cagA-positive strains than in cagA-negative strains. A low G+C content of these two genes suggests they were acquired by horizontal transfer events.

MeSH Terms (8)

Antigens, Bacterial Bacterial Proteins Biomarkers Gene Transfer, Horizontal Genome, Bacterial Genomic Islands Helicobacter pylori Polymerase Chain Reaction

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