Bax-inhibiting peptide derived from mouse and rat Ku70.

Yoshida T, Tomioka I, Nagahara T, Holyst T, Sawada M, Hayes P, Gama V, Okuno M, Chen Y, Abe Y, Kanouchi T, Sasada H, Wang D, Yokota T, Sato E, Matsuyama S
Biochem Biophys Res Commun. 2004 321 (4): 961-6

PMID: 15358121 · DOI:10.1016/j.bbrc.2004.07.054

Bax is a proapoptotic protein that plays a key role in the induction of apoptosis. Ku70 has activities to repair DNA damage in the nucleus and to suppress apoptosis by inhibiting Bax in the cytosol. We previously designed peptides based on the amino acid sequence of Bax-binding domain of human Ku70, and showed that these peptides bind Bax and inhibit cell death in human cell lines. In the present report, we examined the biological activities of other pentapeptides, VPTLK and VPALR, derived from mouse and rat Ku70. Cells in culture accumulated FITC-labeled VPTLK and VPALR, indicating that these peptides are cell permeable (human, mouse, rat, and porcine cells were examined). These peptides bound to Bax and suppressed cell death in various cell types including primary cultured cells. These data suggest that such Bax inhibiting peptides from three mammalian species may be used to protect healthy cells from apoptotic injury under pathological conditions.

MeSH Terms (20)

Amino Acid Sequence Animals Antigens, Nuclear Apoptosis bcl-2-Associated X Protein Cell Line Cells, Cultured DNA-Binding Proteins Etoposide Female Humans Ku Autoantigen Mice Molecular Sequence Data Oligopeptides Ovary Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Rats Sequence Homology, Amino Acid

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