Interaction of Gbetagamma with RACK1 and other WD40 repeat proteins.

Chen S, Spiegelberg BD, Lin F, Dell EJ, Hamm HE
J Mol Cell Cardiol. 2004 37 (2): 399-406

PMID: 15276010 · DOI:10.1016/j.yjmcc.2004.04.019

Heterotrimeric G-proteins, composed of Galpha and Gbetagamma subunits, transmit numerous and diverse extracellular stimuli via a large family of heptahelical cell-surface receptors to various intracellular effector molecules. The Gbetagamma subunit plays a central role in G-protein signaling. The Gbeta subunit belongs to a large family of WD40 repeat proteins, which adopt a circular beta-bladed propeller structure. This unique structural feature confers interactions of Gbetagamma with a variety of proteins to play diverse functions. Intriguingly, we recently found that Gbetagamma can interact with three other WD40 repeat proteins, receptor for activated C kinase 1 (RACK1), dynein intermediate chain-1A and a protein of unknown function. This raises the following questions: are interactions among WD40 proteins a common theme and does the formation of a WD40-WD40 repeat protein complex constitute a protein scaffold for integrating signals from different cellular processes. We are beginning to address these issues by studying the interaction between Gbetagamma and RACK1. Here we will describe the molecular mechanism underlying this interaction and the implications of the interaction on the signal transduction of G-protein and RACK1.

MeSH Terms (14)

Amino Acid Sequence Animals GTP-Binding Protein alpha Subunits GTP-Binding Protein beta Subunits GTP-Binding Protein gamma Subunits Heart Humans Microfilament Proteins Molecular Sequence Data Protein Conformation Receptors, Cell Surface Receptors for Activated C Kinase Repetitive Sequences, Amino Acid Signal Transduction

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