The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.

Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H
Cell. 2004 118 (2): 187-202

PMID: 15260989 · DOI:10.1016/j.cell.2004.06.026

In cells lacking centrosomes, such as those found in female meiosis, chromosomes must nucleate and stabilize microtubules in order to form a bipolar spindle. Here we report the identification of Dasra A and Dasra B, two new components of the vertebrate chromosomal passenger complex containing Incenp, Survivin, and the kinase Aurora B, and demonstrate that this complex is required for chromatin-induced microtubule stabilization and spindle formation. The failure of microtubule stabilization caused by depletion of the chromosomal passenger complex was rescued by codepletion of the microtubule-depolymerizing kinesin MCAK, whose activity is negatively regulated by Aurora B. By contrast, we present evidence that the Ran-GTP pathway of chromatin-induced microtubule nucleation does not require the chromosomal passenger complex, indicating that the mechanisms of microtubule assembly by these two pathways are distinct. We propose that the chromosomal passenger complex regulates local MCAK activity to permit spindle formation via stabilization of chromatin-associated microtubules.

MeSH Terms (28)

Amino Acid Sequence Animals Aurora Kinase B Aurora Kinases Base Sequence Cell Division Cell Extracts Centromere Chromatin Chromosomal Proteins, Non-Histone Chromosome Structures DNA, Complementary HeLa Cells Humans Inhibitor of Apoptosis Proteins Kinesin Macromolecular Substances Microtubule-Associated Proteins Microtubules Molecular Sequence Data Neoplasm Proteins Protein-Serine-Threonine Kinases ran GTP-Binding Protein Sequence Homology, Nucleic Acid Spindle Apparatus Survivin Xenopus Xenopus Proteins

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