Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein.

Huang Q, Yu L, Petros AM, Gunasekera A, Liu Z, Xu N, Hajduk P, Mack J, Fesik SW, Olejniczak ET
Biochemistry. 2004 43 (20): 6059-63

PMID: 15147189 · DOI:10.1021/bi036155b

The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.

MeSH Terms (13)

Animals Binding Sites Humans Ligands Models, Molecular Molecular Sequence Data Molecular Structure Nuclear Magnetic Resonance, Biomolecular Nucleocapsid Proteins Protein Folding Protein Structure, Tertiary RNA SARS Virus

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