Isolation and molecular characterization of a novel type 3 reovirus from a child with meningitis.

Tyler KL, Barton ES, Ibach ML, Robinson C, Campbell JA, O'Donnell SM, Valyi-Nagy T, Clarke P, Wetzel JD, Dermody TS
J Infect Dis. 2004 189 (9): 1664-75

PMID: 15116303 · DOI:10.1086/383129

Mammalian reoviruses are non-enveloped viruses that contain a segmented, double-stranded RNA genome. Reoviruses infect most mammalian species, although infection with these viruses in humans is usually asymptomatic. We report the isolation of a novel reovirus strain from a 6.5-week-old child with meningitis. Hemagglutination and neutralization assays indicated that the isolate is a serotype 3 strain, leading to the designation T3/Human/Colorado/1996 (T3C/96). Sequence analysis of the T3C/96 S1 gene segment, which encodes the viral attachment protein, sigma 1, confirmed the serotype assignment for this strain and indicated that T3C/96 is a novel reovirus isolate. T3C/96 is capable of systemic spread in newborn mice after peroral inoculation and produces lethal encephalitis. These results suggest that serotype 3 reoviruses can cause meningitis in humans.

MeSH Terms (17)

Amino Acid Sequence Animals Animals, Newborn Capsid Proteins Cell Line Cerebrospinal Fluid Female HeLa Cells Humans Infant Mammalian orthoreovirus 3 Meningitis, Viral Mice Molecular Sequence Data Reoviridae Infections Sequence Analysis, DNA Serotyping

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