Production of hydroxyeicosatetraenoic acids and prostaglandins by a novel rat microglial cell line.

Rademacher DJ, Kearn CS, Carrier EJ, Patel S, Delgado MA, Barkmeier A, Klick DE, Breese NM, Pfister SL, Nithipatikom K, Campbell WB, Hillard CJ
J Neuroimmunol. 2004 149 (1-2): 130-41

PMID: 15020073 · DOI:10.1016/j.jneuroim.2003.12.022

We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.

MeSH Terms (24)

Animals Animals, Newborn Blotting, Western Cell Differentiation Cell Line Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Enzyme Inhibitors Glial Fibrillary Acidic Protein Hydroxyeicosatetraenoic Acids Immunohistochemistry Lectins Macrophage Colony-Stimulating Factor Mass Spectrometry Microglia Prostaglandin-Endoperoxide Synthases Prostaglandins Rats Rats, Sprague-Dawley Thymidine Time Factors Tritium Tumor Necrosis Factor-alpha

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