Elucidation of catalytic specificities of human cytochrome P450 and glutathione S-transferase enzymes and relevance to molecular epidemiology.

Guengerich FP, Shimada T, Raney KD, Yun CH, Meyer DJ, Ketterer B, Harris TM, Groopman JD, Kadlubar FF
Environ Health Perspect. 1992 98: 75-80

PMID: 1486866 · PMCID: PMC1519604 · DOI:10.1289/ehp.929875

A number of different approaches have been used to determine the catalytic selectivity of individual human enzymes toward procarcinogens. Studies with cytochrome P450 (P450) enzymes and glutathione S-transferases are summarized here, and recent work with pyrrolizidine alkaloids, aflatoxins, 4,4'-methylenebis(2-chloroaniline), and ethyl carbamate is discussed. In some cases a single enzyme can catalyze both activation and detoxication reactions of a chemical. The purification and characterization of human lung P4501A1 and the development of a noninvasive assay for human P4502E1 are also described.

MeSH Terms (13)

Aflatoxin B1 Biological Assay Carcinogens Catalysis Cytochrome P-450 CYP2E1 Cytochrome P-450 Enzyme System Glutathione Transferase Humans Methylenebis(chloroaniline) Oxidation-Reduction Oxidoreductases, N-Demethylating Pyrrolizidine Alkaloids Urethane

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