Lysine peroxycarbamates: free radical-promoted peptide cleavage.

Masterson DS, Yin H, Chacon A, Hachey DL, Norris JL, Porter NA
J Am Chem Soc. 2004 126 (3): 720-1

PMID: 14733538 · DOI:10.1021/ja038615u

Strategies are reported that combine in one step a predictable chemical-based protein digestion with mass spectrometry. Lysine residue amino groups in peptides and proteins are modified by reaction with a peroxycarbonate derived from p-nitrophenol, and tert-butyl hydroperoxide. The peroxycarbonate reacts with lysine residues in peptides and proteins, and the resulting lysine peroxycarbamates undergo homolytic fragmentation under conditions of low-energy collision-induced dissociation (CID). Observed fragmentation of the peptides involves apparent free radical processes including Hofmann-Löffler-type rearrangements that lead to peptide chain fragmentation. Strategies for directed cleavage of peptides by free radical promoted processes are feasible, and such strategies may well simplify schemes for protein analysis.

MeSH Terms (10)

Amino Acid Sequence Carbamates Free Radicals Lysine Molecular Sequence Data Peptide Mapping Peptides Peroxides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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