TAP1 mutant mice are deficient in antigen presentation, surface class I molecules, and CD4-8+ T cells.

Van Kaer L, Ashton-Rickardt PG, Ploegh HL, Tonegawa S
Cell. 1992 71 (7): 1205-14

PMID: 1473153 · DOI:10.1016/s0092-8674(05)80068-6

The transporter associated with the antigen processing 1 (TAP1) gene encodes a subunit for a transporter, presumed to be involved in the delivery of peptides across the endoplasmic reticulum membrane to class I molecules. We have generated mice with a disrupted TAP1 gene using embryonic stem cell technology. TAP1-deficient mice are defective in the stable assembly and intracellular transport of class I molecules and consequently show severely reduced levels of surface class I molecules. These properties are strikingly similar to those described for the TAP2 mutant cell line RMA-S. Cells from the TAP1-deficient mice are unable to present cytosolic antigens to class I-restricted cytotoxic T cells. As predicted from the near absence of class I surface expression, TAP1-deficient mice lack CD4-8+ T cells.

MeSH Terms (12)

Animals Base Sequence CD4 Antigens CD8 Antigens Cell Line Chimera Gene Expression Regulation Genes, MHC Class I Mice Mice, Transgenic Molecular Sequence Data T-Lymphocytes

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