Global expression analysis of gene regulatory pathways during endocrine pancreatic development.

Gu G, Wells JM, Dombkowski D, Preffer F, Aronow B, Melton DA
Development. 2004 131 (1): 165-79

PMID: 14660441 · DOI:10.1242/dev.00921

To define genetic pathways that regulate development of the endocrine pancreas, we generated transcriptional profiles of enriched cells isolated from four biologically significant stages of endocrine pancreas development: endoderm before pancreas specification, early pancreatic progenitor cells, endocrine progenitor cells and adult islets of Langerhans. These analyses implicate new signaling pathways in endocrine pancreas development, and identified sets of known and novel genes that are temporally regulated, as well as genes that spatially define developing endocrine cells from their neighbors. The differential expression of several genes from each time point was verified by RT-PCR and in situ hybridization. Moreover, we present preliminary functional evidence suggesting that one transcription factor encoding gene (Myt1), which was identified in our screen, is expressed in endocrine progenitors and may regulate alpha, beta and delta cell development. In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation.

MeSH Terms (19)

Animals Cell Differentiation DNA-Binding Proteins Embryonic and Fetal Development Female Gene Expression Regulation, Developmental Green Fluorescent Proteins In Situ Hybridization Islets of Langerhans Luminescent Proteins Mice Mice, Inbred ICR Mice, Transgenic Pregnancy Protein-Serine-Threonine Kinases Protein-Tyrosine Kinases Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Transcription Factors

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