Repeated alpha-galactosylceramide administration results in expansion of NK T cells and alleviates inflammatory dermatitis in MRL-lpr/lpr mice.

Yang JQ, Saxena V, Xu H, Van Kaer L, Wang CR, Singh RR
J Immunol. 2003 171 (8): 4439-46

PMID: 14530371 · DOI:10.4049/jimmunol.171.8.4439

NK T (NKT) cells expressing the invariant Valpha14-Jalpha18 TCR alpha-chain recognize glycolipid Ags such as alpha-galactosylceramide (alpha-GalCer) presented by the MHC class I-like molecule CD1d. Upon activation by alpha-GalCer, invariant NKT cells secrete multiple cytokines and confer protection in certain immune-mediated disorders. Here we have investigated the role of NKT cells in the development of inflammatory dermatitis in MRL-lpr/lpr mice, which shares features with lupus in humans. Our results show that the numbers Sand functions of NKT (TCRbeta(+)CD1d/alpha-GalCer tetramer(+)) cells, particularly of the NK1.1(-) subset, are reduced in MRL-lpr/lpr mice compared with MRL-fas/fas and/or nonautoimmune C3H/Hej and BALB/c mice. Repeated treatments with alpha-GalCer result in the expansion of NKT cells and alleviate dermatitis in MRL-lpr/lpr mice. Our results indicate that NKT cell deficiency can be corrected by repeated alpha-GalCer treatment and that NKT cells may play a protective role in inflammatory dermatitis of lupus-prone mice.

MeSH Terms (18)

Animals Cell Division Dermatitis Drug Administration Schedule Female Galactosylceramides Genetic Predisposition to Disease Injections, Intraperitoneal Killer Cells, Natural Lupus Nephritis Lymphocyte Activation Lymphocyte Count Lymphopenia Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred MRL lpr T-Lymphocyte Subsets

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