A polymorphic human kidney-specific non-MHC alloantigen. Its possible role in tissue-specific allograft immunity.

Joyce S, Mathew JM, Flye MW, Mohanakumar T
Transplantation. 1992 53 (5): 1119-27

PMID: 1374945 · DOI:10.1097/00007890-199205000-00027

Tissue specific non-MHC alloantigens play a crucial role in allograft immunity. However, their structural properties have remained elusive, largely due to their inability to induce a strong antibody response. We report the characterization of a monkey heteroantiserum, MHK-I, raised against human kidney cells, that serologically reacts specifically with kidney cells after extensive absorptions of anti-HLA class I and II reactivities. The non-MHC MHK-I-binding molecule(s) is expressed only in the renal cortex on the glomerulus, peritubular capillaries, venous endothelium, and tubular epithelium. Immunochemically, MHK-I recognizes a kidney-specific non-MHC alloantigen of Mr 90,000 to 100,000 (90 kD). These properties of MHK-I are similar to those of the previously characterized alloantibodies eluted from rejected kidneys. These alloantibodies bind to the kidney from which the antibody was eluted and to a few others but are unlike MHK-I, which binds to extracts prepared from all human kidneys. Biochemical analysis by two-dimensional electrophoresis (pI ranging between 4.5 and 5.5) and peptide fingerprinting provide further evidence that the alloantigen is polymorphic. These findings imply that the non-MHC kidney-specific molecule(s) may function as target(s) for immune destruction of renal allografts.

MeSH Terms (16)

Animals Blotting, Western Epitopes Graft Rejection Haplorhini Histocompatibility Antigens Humans Immune Sera Immunity Immunohistochemistry Isoantigens Kidney Kidney Transplantation Peptide Mapping Polymorphism, Genetic Transplantation, Homologous

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