Semidry electrophoretic transfer of RNA to membranes.

Lane KB, Zoia O, Davidson JM
Biotechniques. 1992 12 (3): 340-2, 346

PMID: 1373942

We describe a method using a semi-dry gel electro-blotter to transfer RNA from standard agarose-formaldehyde denaturing gels in less than 30 min. The method requires equilibrating the gel in a low ionic strength buffer. The transfer is done under high-current and low-voltage conditions. This method maintains the overall sharpness of the bands on the final autoradiogram while shortening the time required for Northern transfer by approximately 12 hours.

MeSH Terms (9)

Animals Biotechnology Buffers Elastin Electrophoresis, Agar Gel Evaluation Studies as Topic Muscle, Smooth, Vascular RNA Swine

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