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We describe a method using a semi-dry gel electro-blotter to transfer RNA from standard agarose-formaldehyde denaturing gels in less than 30 min. The method requires equilibrating the gel in a low ionic strength buffer. The transfer is done under high-current and low-voltage conditions. This method maintains the overall sharpness of the bands on the final autoradiogram while shortening the time required for Northern transfer by approximately 12 hours.
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Released July 13, 2020