A Na pump inhibitor from bovine posterior pituitary: purification, structure determination and its cardiovascular effect in rat.

Hamakubo T, Furuta H, Ichimura M, Appalsamy M, Mosqueda-Garcia R, Robertson D, Inagami T
Biochem Biophys Res Commun. 1992 189 (2): 691-6

PMID: 1335244 · DOI:10.1016/0006-291x(92)92256-w

We examined the hypothesis that hypothalamo-hypophysial tissue contains an endogenous Na pump inhibitor. From bovine posterior pituitary, we purified a substance which inhibits Rb uptake by human erythrocytes. This inhibitory activity was found in the eluate of 10% acetonitrile from a C18 flash column and purified by subsequent three steps of reversed-phase high-performance liquid chromatography (HPLC). Sequence analysis revealed that this substance was identical to joining peptide, one of the major products of proopiomelanocortin (POMC). This peptide had hypertensive and tachycardiac effects in spontaneously hypertensive rats (SHR) after central administration, with weak Na,K-ATPase inhibitory activity (IC50 = 0.5 mM).

MeSH Terms (21)

Amino Acid Sequence Animals Biological Transport Blood Pressure Cattle Chromatography Chromatography, High Pressure Liquid Erythrocytes Heart Rate Humans Male Molecular Sequence Data Peptide Fragments Peptides Pituitary Gland, Posterior Pro-Opiomelanocortin Rats Rats, Inbred SHR Rats, Inbred WKY Rubidium Sodium-Potassium-Exchanging ATPase

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