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Superoxide generation by the human polymorphonuclear leukocyte in response to latex beads.

Thomas MJ, Hedrick CC, Smith S, Pang J, Jerome WG, Willard AS, Shirley PS
J Leukoc Biol. 1992 51 (6): 591-6

PMID: 1319445 · DOI:10.1002/jlb.51.6.591

In this study, superoxide formation was not immediately detected when polymorphonuclear leukocytes were treated with linoleyl alcohol-coated, carboxy-modified latex beads. However, all other measures of neutrophil activation were present. Superoxide was not detected until 30 min after the initial exposure to beads. However, an O2(-)-producing, NADPH-dependent oxidase is active 15 min after exposure. When polymorphonuclear leukocytes are pretreated with cytochalasin B, superoxide production is detected immediately after exposure to the beads. Superoxide secretion after treatment with linoleyl alcohol-coated latex beads is compared with the response to other latex beads. The results imply that neutrophils form a phagolysosome around linoleyl alcohol-coated latex beads that is tightly sealed and does not allow superoxide to escape into the medium where it could be detected by the reduction of ferricytochrome c.

MeSH Terms (7)

Cells, Cultured Humans Latex NADH, NADPH Oxidoreductases NADPH Oxidases Neutrophils Superoxides

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