A Hox 3.3-lacZ transgene expressed in developing limbs.

Jegalian BG, Miller RW, Wright CV, Blum M, De Robertis EM
Mech Dev. 1992 39 (3): 171-80

PMID: 1292571

We describe transgenic mouse lines that express lacZ under the control of the Hox 3.3 Promoter II. The correct anterior boundary can be fixed by 3.6 kb of promoter DNA (plus 1.6 kb of 5' transcribed sequences), both in tissues of ectodermal and mesodermal origin. The posterior border, however, is not respected, and lacZ expression continues into the tail region. One line has particularly strong graded expression in the anterior proximal limb bud. Other lines, containing a shorter promoter fragment (0.6 kb), have ectopic expression in the head region, including one line that has expression in the anterior half of the retina. Such mouse lines make it possible to molecularly distinguish cells in regions of the embryo that look otherwise identical and may be useful in studying the establishment of molecular differences in the mouse embryo.

MeSH Terms (13)

Animals Brain Chromosome Mapping DNA-Binding Proteins Extremities Gene Expression Regulation Genes Homeodomain Proteins Lac Operon Mice Mice, Transgenic Promoter Regions, Genetic Retina

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