Collecting duct-specific gene inactivation of alphaENaC in the mouse kidney does not impair sodium and potassium balance.

Rubera I, Loffing J, Palmer LG, Frindt G, Fowler-Jaeger N, Sauter D, Carroll T, McMahon A, Hummler E, Rossier BC
J Clin Invest. 2003 112 (4): 554-65

PMID: 12925696 · PMCID: PMC171384 · DOI:10.1172/JCI16956

Aldosterone controls the final sodium reabsorption and potassium secretion in the kidney by regulating the activity of the epithelial sodium channel (ENaC) in the aldosterone-sensitive distal nephron (ASDN). ASDN consists of the last portion of the distal convoluted tubule (late DCT), the connecting tubule (CNT), and the collecting duct (CD) (i.e., the cortical CD [CCD] and the medullary CD [MCD]). It has been proposed that the control of sodium transport in the CCD is essential for achieving sodium and potassium balance. We have tested this hypothesis by inactivating the alpha subunit of ENaC in the CD but leaving ENaC expression in the late DCT and CNT intact. Under salt restriction or under aldosterone infusion, whole-cell voltage clamp of principal cells of CCD showed no detectable ENaC activity, whereas large amiloride-sensitive currents were observed in control littermates. The animals survive well and are able to maintain sodium and potassium balance, even when challenged by salt restriction, water deprivation, or potassium loading. We conclude that the expression of ENaC in the CD is not a prerequisite for achieving sodium and potassium balance in mice. This stresses the importance of more proximal nephron segments (late DCT/CNT) to achieve sodium and potassium balance.

MeSH Terms (20)

Aldosterone Animals Electrophysiology Epithelial Sodium Channels Homeodomain Proteins Immunohistochemistry Kidney Tubules Kidney Tubules, Collecting Mice Mice, Knockout Microscopy, Fluorescence Potassium Promoter Regions, Genetic Protein Structure, Tertiary RNA, Messenger Salts Sodium Sodium Channels Time Factors Water

Connections (1)

This publication is referenced by other Labnodes entities:

Links