Flow cytometric analysis of aneuploidy and S-phase fraction in chronic myeloid leukemia patients: role in early detection of accelerated phase.

Tripathi AK, Chaturvedi R, Ahmad R, Asim M, Sawlani KK, Singh MK, Tripathi P, Tekwani BL
Leuk Res. 2003 27 (10): 899-902

PMID: 12860009 · DOI:10.1016/s0145-2126(03)00025-0

We studied S-phase fraction (SPF) and aneuploidy in peripheral blood leucocytes of patients with chronic myeloid leukemia (CML) in chronic phase (CML-CP, n=41), accelerated phase (CML-AP, n=6), and control subjects (n=12) with an aim to find out their role in early detection of accelerated phase. The SPF and aneuploidy were studied through flow-cytometry using LT-Mod. Fit software. Mean SPF value in CML-AP (9.28+/-3.46%) and in CML-CP (4.76+/-2.30%) were significantly higher than in normal controls (0.28+/-0.21%), (P<0.005, P<0.001). CML-CP patients having higher SPF (>7%) converted to accelerated phase within 18 months of follow-up while those with lower SPF (<7%) did not. Aneuploidy was present in 34.14% of CML-CP and all patients of CML-AP whereas no control subjects showed aneuploidy. Among CML-CP patients having SPF >7%, 86% developed aneuploidy during follow-up as compared to 18.50% of CML-CP with less than 7% SPF. We conclude that peripheral blood SPF and aneuploidy could be important parameters for prediction of evolution to accelerated phase in CML patients.

MeSH Terms (14)

Adult Aneuploidy Case-Control Studies Disease Progression Female Flow Cytometry Follow-Up Studies Humans Leukemia, Myelogenous, Chronic, BCR-ABL Positive Leukocytes Male Middle Aged Predictive Value of Tests S Phase

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