Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experiments.

Logan TM, Olejniczak ET, Xu RX, Fesik SW
FEBS Lett. 1992 314 (3): 413-8

PMID: 1281793 · DOI:10.1016/0014-5793(92)81517-p

Two multi-dimensional heteronuclear NMR experiments are described for assigning the resonances in uniformly 15N- and 13C-labeled proteins. In one experiment (HCNH-TOCSY), the amide nitrogen and proton are correlated to the side-chain protons and carbons of the same and preceding residue. In a second triple resonance experiment (HC(CO)NH-TOCSY), the amide nitrogen and proton of one residue is correlated exclusively with the side-chain proton and carbon resonances of the preceding residue by transferring magnetization through the intervening carbonyl. The utility of these two experiments for making sequential resonance assignments in proteins is illustrated for [U-15N,13C]FKBP (107 residues) complexed to the immunosuppressant, ascomycin.

MeSH Terms (9)

Amino Acids Carbon Isotopes Carrier Proteins Magnetic Resonance Spectroscopy Nitrogen Nitrogen Isotopes Proteins Tacrolimus Tacrolimus Binding Proteins

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