Signal recognition particle binds to ribosome-bound signal sequences with fluorescence-detected subnanomolar affinity that does not diminish as the nascent chain lengthens.

Flanagan JJ, Chen JC, Miao Y, Shao Y, Lin J, Bock PE, Johnson AE
J Biol Chem. 2003 278 (20): 18628-37

PMID: 12621052 · DOI:10.1074/jbc.M300173200

MeSH Terms (21)

Animals Binding, Competitive Cricetinae Dose-Response Relationship, Drug Endoplasmic Reticulum Kinetics Ligands Mesocricetus Models, Chemical Pan troglodytes Plasmids Protein Binding Protein Biosynthesis Protein Transport Rats Ribosomes RNA, Messenger RNA, Transfer Signal Recognition Particle Spectrometry, Fluorescence Thermodynamics

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