Tristetraprolin binds to the 3'-untranslated region of cyclooxygenase-2 mRNA. A polyadenylation variant in a cancer cell line lacks the binding site.

Sawaoka H, Dixon DA, Oates JA, Boutaud O
J Biol Chem. 2003 278 (16): 13928-35

PMID: 12578839 · DOI:10.1074/jbc.M300016200

In human colorectal adenocarcinoma cell lines, we found two major transcripts of cyclooxygenase-2, the full-length mRNA and a short polyadenylation variant (2577 kb) lacking the distal segment of the 3'-untranslated region. Tristetraprolin, an mRNA-binding protein that promotes message instability, was shown to bind the cyclooxygenase-2 mRNA in the region of the 3'-untranslated region between nucleotides 3125 and 3432 and to reduce levels of the full-length mRNA. During cell growth and confluence, the expression of tristetraprolin mRNA was inversely correlated with that of the full-length cyclooxygenase-2 transcript, and transfection of tristetraprolin into HCA-7 cells reduced the level of full-length cyclooxygenase-2 mRNA. However, the truncated transcript escaped tristetraprolin binding and downregulation.

MeSH Terms (34)

3' Untranslated Regions Base Sequence Binding Sites Blotting, Northern Blotting, Western Catalysis Cells, Cultured Cross-Linking Reagents Cyclooxygenase 2 DNA, Complementary DNA-Binding Proteins Dose-Response Relationship, Drug Down-Regulation Endothelium, Vascular Humans Immediate-Early Proteins Isoenzymes Lipopolysaccharides Membrane Proteins Molecular Sequence Data Polyadenylation Precipitin Tests Prostaglandin-Endoperoxide Synthases Protein Binding Ribonucleases RNA RNA, Messenger Time Factors Transcription, Genetic Transfection Tristetraprolin Tumor Cells, Cultured Umbilical Veins Up-Regulation

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