Forces for morphogenesis investigated with laser microsurgery and quantitative modeling.

Hutson MS, Tokutake Y, Chang MS, Bloor JW, Venakides S, Kiehart DP, Edwards GS
Science. 2003 300 (5616): 145-9

PMID: 12574496 · DOI:10.1126/science.1079552

We investigated the forces that connect the genetic program of development to morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for development and wound healing. We found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages. We applied quantitative modeling to show that these forces, generated in distinct cells, are coordinated in space and synchronized in time. Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.

MeSH Terms (21)

Animals Animals, Genetically Modified Cell Adhesion Drosophila Drosophila Proteins Embryo, Nonmammalian Embryonic Development Epithelial Cells Epithelium Genes, Insect Image Processing, Computer-Assisted Integrin alpha Chains Integrins Lasers Mathematics Microscopy, Confocal Microsurgery Models, Biological Morphogenesis Mutation Pseudopodia

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