Prosurvival and antiapoptotic effects of PGE2 in radiation injury are mediated by EP2 receptor in intestine.

Houchen CW, Sturmoski MA, Anant S, Breyer RM, Stenson WF
Am J Physiol Gastrointest Liver Physiol. 2003 284 (3): G490-8

PMID: 12431904 · DOI:10.1152/ajpgi.00240.2002

The biological activities of PGE(2) are mediated through EP receptors (EP(1)-EP(4)), plasma membrane G protein-coupled receptors that differ in ligand binding and signal-transduction pathways. We investigated gastrointestinal EP(2) receptor expression in adult mice before and after radiation injury and evaluated intestinal stem cell survival and crypt epithelial apoptosis after radiation injury in EP(2) null mice. EP(2) was expressed throughout the gut. Intestinal EP(2) mRNA increased fivefold after gamma-irradiation. Crypt survival was diminished in EP(2)-/- mice (4.06 crypts/cross section) compared with wild-type littermates (8.15 crypts/cross section). Radiation-induced apoptosis was significantly increased in EP(2)-/- mice compared with wild-type littermates. Apoptosis was 1.6-fold higher in EP(2) (-/-) mice (5.9 apoptotic cells/crypt) than in wild-type mice (3.5 apoptotic cells/crypt). The EP(2) receptor is expressed in mouse gastrointestinal epithelial cells and is upregulated following radiation injury. The effects of PGE(2) on both crypt epithelial apoptosis and intestinal crypt stem cell survival are mediated through the EP(2) receptor.

MeSH Terms (15)

Animals Apoptosis Blotting, Western Cell Survival Dinoprostone Electrophoresis, Polyacrylamide Gel Epithelial Cells Immunohistochemistry Intestines Mice Radiation Injuries, Experimental Receptors, Prostaglandin E Receptors, Prostaglandin E, EP2 Subtype Receptors, Prostaglandin E, EP4 Subtype RNA, Messenger

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