Hepatocyte nuclear factor-4 alpha mediates the stimulatory effect of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) on glucose-6-phosphatase catalytic subunit gene transcription in H4IIE cells.

Boustead JN, Stadelmaier BT, Eeds AM, Wiebe PO, Svitek CA, Oeser JK, O'Brien RM
Biochem J. 2003 369 (Pt 1): 17-22

PMID: 12416993 · PMCID: PMC1223073 · DOI:10.1042/BJ20021382

It has recently been shown that adenoviral-mediated expression of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) in hepatocytes stimulates glucose-6-phosphatase catalytic subunit (G6Pase) gene expression. A combination of fusion gene, gel retardation and chromatin immunoprecipitation assays revealed that, in H4IIE cells, PGC-1 alpha mediates this stimulation through an evolutionarily conserved region of the G6Pase promoter that binds hepatocyte nuclear factor-4 alpha.

MeSH Terms (20)

Animals Base Sequence Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Binding Sites Catalytic Domain DNA-Binding Proteins DNA Primers Gene Expression Regulation, Enzymologic Glucose-6-Phosphatase Hepatocyte Nuclear Factor 4 Mice Phosphoproteins Precipitin Tests Promoter Regions, Genetic Protein Binding Receptors, Cytoplasmic and Nuclear Sequence Deletion Transcription, Genetic Transcription Factors Tumor Cells, Cultured

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