Evaluation of the murine immune response to Leishmania meta 1 antigen delivered as recombinant protein or DNA vaccine.

Serezani CH, Franco AR, Wajc M, Umada Yokoyama-Yasunaka JK, Wunderlich G, Borges MM, Uliana SR
Vaccine. 2002 20 (31-32): 3755-63

PMID: 12399206 · DOI:10.1016/s0264-410x(02)00354-7

The meta 1 gene of Leishmania is conserved across the genus and encodes a protein upregulated in metacyclic promastigotes. Meta 1 constitutive overexpressing mutants show increased virulence to mice. In this paper, both meta 1 recombinant protein and plasmids bearing the meta 1 gene were tested for their antigenicity and potential for inducing protective immunity in mice. Vaccination with the recombinant protein induced a predominant Th2-type of response and did not result in protection upon challenge with live parasites. Surprisingly, the expected reversal to a CD4(+) Th1-type of response upon genetic immunisation by the intramuscular route was not observed. Instead, vaccination with either the meta 1 gene alone or in fusion with the monocyte chemotactic protein (MCP)-3 cDNA induced a Th2-type of response that correlated with lack of protection against infection.

MeSH Terms (24)

Animals Antibodies, Protozoan Cell Line Cercopithecus aethiops Chemokine CCL7 COS Cells Cytokines Female Leishmania major Leishmania mexicana Leishmaniasis, Cutaneous Leishmaniasis, Diffuse Cutaneous Mice Mice, Inbred BALB C Monocyte Chemoattractant Proteins Protozoan Proteins Protozoan Vaccines Recombinant Proteins Solubility T-Lymphocyte Subsets Th1 Cells Th2 Cells Vaccines, DNA Vaccines, Synthetic

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