Evaluation of Tat-encoding bicistronic human immunodeficiency virus type 1 gene transfer vectors in primary canine bone marrow mononuclear cells.

Srinivasakumar N, Zaboikin M, Zaboikina T, Schuening F
J Virol. 2002 76 (14): 7334-42

PMID: 12072532 · PMCID: PMC136335 · DOI:10.1128/jvi.76.14.7334-7342.2002

Tat-encoding human immunodeficiency virus type 1 (HIV-1) gene transfer vectors were evaluated in primary canine bone marrow mononuclear cells. Tat vectors provided higher levels of gene expression than vectors with internal promoters. The HIV-1 vector was also more efficient than Moloney murine leukemia virus (MoMLV) vectors for transduction of canine bone marrow mononuclear cells in vitro. Transplantation experiments in dogs with transduced autologous marrow cells confirmed the superiority of HIV-1 vectors over MoMLV vectors for gene transfer into canine bone marrow cells. Tat vectors may be useful not only for providing high levels of therapeutic gene expression in hematopoietic cells but also for study of the biological effects of Tat in those tissues in the canine model.

MeSH Terms (15)

Animals Bone Marrow Cells Bone Marrow Transplantation Dogs Flow Cytometry Gene Products, tat Genetic Vectors Gene Transfer Techniques Green Fluorescent Proteins HIV-1 Humans Leukemia Virus, Murine Luminescent Proteins tat Gene Products, Human Immunodeficiency Virus Transduction, Genetic

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