PI 3-kinase regulation of dopamine uptake.

Carvelli L, MorĂ³n JA, Kahlig KM, Ferrer JV, Sen N, Lechleiter JD, Leeb-Lundberg LM, Merrill G, Lafer EM, Ballou LM, Shippenberg TS, Javitch JA, Lin RZ, Galli A
J Neurochem. 2002 81 (4): 859-69

PMID: 12065645 · DOI:10.1046/j.1471-4159.2002.00892.x

The magnitude and duration of dopamine (DA) signaling is defined by the amount of vesicular release, DA receptor sensitivity, and the efficiency of DA clearance, which is largely determined by the DA transporter (DAT). DAT uptake capacity is determined by the number of functional transporters on the cell surface as well as by their turnover rate. Here we show that inhibition of phosphatidylinositol (PI) 3-kinase with LY294002 induces internalization of the human DAT (hDAT), thereby reducing transport capacity. Acute treatment with LY294002 reduced the maximal rate of [(3) H]DA uptake in rat striatal synaptosomes and in human embryonic kidney (HEK) 293 cells stably expressing the hDAT (hDAT cells). In addition, LY294002 caused a significant redistribution of the hDAT from the plasma membrane to the cytosol. Conversely, insulin, which activates PI 3-kinase, increased [(3)H]DA uptake and blocked the amphetamine-induced hDAT intracellular accumulation, as did transient expression of constitutively active PI 3-kinase. The LY294002-induced reduction in [(3)H]DA uptake and hDAT cell surface expression was inhibited by expression of a dominant negative mutant of dynamin I, indicating that dynamin-dependent trafficking can modulate transport capacity. These data implicate DAT trafficking in the hormonal regulation of dopaminergic signaling, and suggest that a state of chronic hypoinsulinemia, such as in diabetes, may alter synaptic DA signaling by reducing the available cell surface DATs.

MeSH Terms (30)

Amphetamine Animals Biological Transport Cell Line Cell Membrane Chromones Corpus Striatum Cytosol Dopamine Dopamine Plasma Membrane Transport Proteins Dose-Response Relationship, Drug Dynamin I Dynamins Enzyme Inhibitors Genes, Dominant GTP Phosphohydrolases Humans Insulin Kidney Male Membrane Glycoproteins Membrane Transport Proteins Morpholines Nerve Tissue Proteins Phosphatidylinositol 3-Kinases Protein Transport Rats Recombinant Fusion Proteins Synaptosomes Transfection

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