Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.

Saitoh S, Chabes A, McDonald WH, Thelander L, Yates JR, Russell P
Cell. 2002 109 (5): 563-73

PMID: 12062100 · DOI:10.1016/s0092-8674(02)00753-5

Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate all mRNA, Cid13 is a cytoplasmic enzyme that specifically targets suc22 mRNA that encodes a subunit of ribonucleotide reductase (RNR). cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance.

MeSH Terms (23)

Cell Nucleus Chromosomal Proteins, Non-Histone Cytoplasm DNA DNA-Directed DNA Polymerase DNA-Directed RNA Polymerases DNA Replication Eukaryotic Cells Fungal Proteins Gene Expression Regulation, Enzymologic Genetic Testing Genomic Library Hydroxyurea Molecular Sequence Data Mutation Nuclear Proteins Polynucleotide Adenylyltransferase Ribonucleotide Reductases RNA, Messenger RNA-Binding Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid

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