Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD.

Du Q, Carrasco N, Teplova M, Wilds CJ, Egli M, Huang Z
J Am Chem Soc. 2002 124 (1): 24-5

PMID: 11772055 · DOI:10.1021/ja0171097

We have developed a route for the synthesis of 2'-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.

MeSH Terms (6)

Crystallography, X-Ray DNA Oligonucleotides Organoselenium Compounds RNA Spectrometry, Fluorescence

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