Mus81-Eme1 are essential components of a Holliday junction resolvase.

Boddy MN, Gaillard PH, McDonald WH, Shanahan P, Yates JR, Russell P
Cell. 2001 107 (4): 537-48

PMID: 11719193 · DOI:10.1016/s0092-8674(01)00536-0

Mus81, a fission yeast protein related to the XPF subunit of ERCC1-XPF nucleotide excision repair endonuclease, is essential for meiosis and important for coping with stalled replication forks. These processes require resolution of X-shaped DNA structures known as Holliday junctions. We report that Mus81 and an associated protein Eme1 are components of an endonuclease that resolves Holliday junctions into linear duplex products. Mus81 and Eme1 are required during meiosis at a late step of meiotic recombination. The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Eme1 are subunits of a nuclear Holliday junction resolvase.

MeSH Terms (24)

Amino Acid Sequence Binding Sites DNA, Fungal DNA-Binding Proteins DNA Damage DNA Repair Endodeoxyribonucleases Endonucleases Escherichia coli Proteins Holliday Junction Resolvases Macromolecular Substances Meiosis Models, Genetic Models, Molecular Molecular Sequence Data Protein Binding Recombinant Fusion Proteins Recombination, Genetic Saccharomyces cerevisiae Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Substrate Specificity Transfection

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