Lsh, a member of the SNF2 family, is required for genome-wide methylation.

Dennis K, Fan T, Geiman T, Yan Q, Muegge K
Genes Dev. 2001 15 (22): 2940-4

PMID: 11711429 · PMCID: PMC312825 · DOI:10.1101/gad.929101

Methylation patterns of the mammalian genome are thought to be crucial for development. The precise mechanisms designating specific genomic loci for methylation are not known. Targeted deletion of Lsh results in perinatal lethality with a rather normal development. We report here, however, that Lsh(-/-) mice show substantial loss of methylation throughout the genome. The hypomethylated loci comprise repetitive elements and single copy genes. This suggests that global genomic methylation is not absolutely required for normal embryogenesis. Based on the similarity of Lsh to other SNF2 chromatin remodeling proteins, it suggests that alteration of chromatin affects global methylation patterns in mice.

MeSH Terms (19)

Animals Blotting, Southern Chromatin DNA-Binding Proteins DNA Helicases DNA Methylation Genome Genomic Imprinting Genotype Globins Isoenzymes Mice Multigene Family Nuclear Proteins Phosphoglycerate Kinase Protein Structure, Tertiary Reverse Transcriptase Polymerase Chain Reaction Tissue Distribution Transcription Factors

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