Reovirus-induced sigma1s-dependent G(2)/M phase cell cycle arrest is associated with inhibition of p34(cdc2).

Poggioli GJ, Dermody TS, Tyler KL
J Virol. 2001 75 (16): 7429-34

PMID: 11462015 · PMCID: PMC114978 · DOI:10.1128/JVI.75.16.7429-7434.2001

Serotype 3 reoviruses inhibit cellular proliferation by inducing a G(2)/M phase cell cycle arrest. Reovirus-induced G(2)/M phase arrest requires the viral S1 gene-encoded sigma1s nonstructural protein. The G(2)-to-M transition represents a cell cycle checkpoint that is regulated by the kinase p34(cdc2). We now report that infection with serotype 3 reovirus strain Abney, but not serotype 1 reovirus strain Lang, is associated with inhibition and hyperphosphorylation of p34(cdc2). The sigma1s protein is necessary and sufficient for inhibitory phosphorylation of p34(cdc2), since a viral mutant lacking sigma1s fails to hyperphosphorylate p34(cdc2) and inducible expression of sigma1s is sufficient for p34(cdc2) hyperphosphorylation. These studies establish a mechanism by which reovirus can perturb cell cycle regulation.

MeSH Terms (10)

Animals CDC2 Protein Kinase Cell Cycle Cell Line Mammalian orthoreovirus 3 Mice Reoviridae Infections Signal Transduction Viral Nonstructural Proteins Virus Replication

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