The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors.

Reck-Peterson SL, Tyska MJ, Novick PJ, Mooseker MS
J Cell Biol. 2001 153 (5): 1121-6

PMID: 11381095 · PMCID: PMC2174330 · DOI:10.1083/jcb.153.5.1121

The motor properties of the two yeast class V myosins, Myo2p and Myo4p, were examined using in vitro motility assays. Both myosins are active motors with maximum velocities of 4.5 microm/s for Myo2p and 1.1 microm/s for Myo4p. Myo2p motility is Ca(2+) insensitive. Both myosins have properties of a nonprocessive motor, unlike chick myosin-Va (M5a), which behaves as a processive motor when assayed under identical conditions. Additional support for the idea that Myo2p is a nonprocessive motor comes from actin cosedimentation assays, which show that Myo2p has a low affinity for F-actin in the presence of ATP and Ca(2+), unlike chick brain M5a. These studies suggest that if Myo2p functions in organelle transport, at least five molecules of Myo2p must be present per organelle to promote directed movement.

MeSH Terms (23)

Actins Adenosine Triphosphate Animals Antibodies Brain Calcium Calmodulin-Binding Proteins Carrier Proteins Chickens Fungal Proteins Kinetics Microscopy, Video Molecular Motor Proteins Movement Myosin Heavy Chains Myosins Myosin Type II Myosin Type V Nerve Tissue Proteins Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins

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