Development and characterization of a molecular viability assay for Pneumocystis carinii f sp hominis.

Maher NH, Vermund SH, Welsh DA, Dillon HK, Awooda A, Unnasch TR
J Infect Dis. 2001 183 (12): 1825-7

PMID: 11372040 · DOI:10.1086/320738

Pneumocystis carinii pneumonia (PCP) remains the most common opportunistic infection among human immunodeficiency virus-infected persons. Despite this, little is known concerning the transmission dynamics of this infection. In the absence of a reliable method to isolate and culture P. carinii from environmental samples, it has not been possible to assess the importance of person-to-person transmission in the epidemiology of PCP. A molecular viability assay was developed for the human form of P. carinii (P. carinii f sp hominis) that is applicable to both clinical specimens and environmental samples. This assay will enable the evaluation of the spread and persistence of viable human P. carinii in the environment.

MeSH Terms (16)

Adult AIDS-Related Opportunistic Infections Bronchoalveolar Lavage Fluid DNA, Bacterial Female Humans Male Middle Aged Molecular Sequence Data Pneumocystis Pneumonia, Pneumocystis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Ribosomal, 18S Sequence Analysis, DNA Sputum

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