Gene expression profiling of malignant mesothelioma cell lines: cDNA array study.

Kettunen E, Nissén AM, Ollikainen T, Taavitsainen M, Tapper J, Mattson K, Linnainmaa K, Knuutila S, El-Rifai W
Int J Cancer. 2001 91 (4): 492-6

PMID: 11251971 · DOI:10.1002/1097-0215(200002)9999:9999<::aid-ijc1094>;2-m

To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.

Copyright 2001 Wiley-Liss, Inc.

MeSH Terms (12)

DNA, Complementary Down-Regulation Electrophoresis, Agar Gel Epithelium Gene Expression Regulation, Neoplastic Humans Mesothelioma Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Up-Regulation

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