Partial purification and properties of calf thymus deoxyribonucleic acid dependent RNA polymerase III.

Weil PA, Blatti SP
Biochemistry. 1975 14 (8): 1636-42

PMID: 1125192 · DOI:10.1021/bi00679a015

DNA-dependent RNA polymerase III (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.-7.6) has been isolated and partially purified from calf thymus tissue. Significant amounts of enzyme III are present in this tissue (up to 15% of the total activity of thymus homogenates). This enzyme has been characterized with respect to its chromatographic properties, broad ammonium sulfate optimum (0.04-0.2 M), template requirements, divalent metal optima, and its unique alpha-amanitin sensitivity (50% inhibition of activity occurring at an alpha-amanitin concentration of 10 mug/ml).

MeSH Terms (15)

Amanitins Ammonium Sulfate Animals Cattle Centrifugation, Density Gradient Chromatography, Ion Exchange Dactinomycin DNA DNA-Directed RNA Polymerases Electrophoresis, Polyacrylamide Gel Isoenzymes Kinetics Solubility Templates, Genetic Thymus Gland

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