Genomic organization and characterization of the white locus of the Mediterranean fruitfly, Ceratitis capitata.

Gomulski LM, Pitts RJ, Costa S, Saccone G, Torti C, Polito LC, Gasperi G, Malacrida AR, Kafatos FC, Zwiebel LJ
Genetics. 2001 157 (3): 1245-55

PMID: 11238408 · PMCID: PMC1461546

An approximately 14-kb region of genomic DNA encoding the wild-type white eye (w+) color gene from the medfly, Ceratitis capitata has been cloned and characterized at the molecular level. Comparison of the intron-exon organization of this locus among several dipteran insects reveals distinct organizational patterns that are consistent with the phylogenetic relationships of these flies and the dendrogram of the predicted primary amino acid sequence of the white loci. An examination of w+ expression during medfly development has been carried out, displaying overall similarity to corresponding studies for white gene homologues in Drosophila melanogaster and other insects. Interestingly, we have detected two phenotypically neutral allelic forms of the locus that have arisen as the result of an apparently novel insertion or deletion event located in the large first intron of the medfly white locus. Cloning and sequencing of two mutant white alleles, w1 and w2, from the we,wp and M245 strains, respectively, indicate that the mutant conditions in these strains are the result of independent events--a frameshift mutation in exon 6 for w1 and a deletion including a large part of exon 2 in the case of w2.

MeSH Terms (25)

Alleles Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Cell Lineage Cloning, Molecular Diptera DNA, Complementary Drosophila melanogaster Exons Gene Deletion Gene Transfer Techniques Genome Introns Models, Genetic Molecular Sequence Data Mutation Photoreceptor Cells, Invertebrate Phylogeny Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid

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