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Impaired adipogenesis and lipolysis in the mouse upon selective ablation of the retinoid X receptor alpha mediated by a tamoxifen-inducible chimeric Cre recombinase (Cre-ERT2) in adipocytes.

Imai T, Jiang M, Chambon P, Metzger D
Proc Natl Acad Sci U S A. 2001 98 (1): 224-8

PMID: 11134524 · PMCID: PMC14572 · DOI:10.1073/pnas.011528898

Retinoid X receptor alpha (RXRalpha) is involved in multiple signaling pathways, as a heterodimeric partner of several nuclear receptors. To investigate its function in energy homeostasis, we have selectively ablated the RXRalpha gene in adipocytes of 4-week-old transgenic mice by using the tamoxifen-inducible Cre-ERT2 recombination system. Mice lacking RXRalpha in adipocytes were resistant to dietary and chemically induced obesity and impaired in fasting-induced lipolysis. Our results also indicate that RXRalpha is involved in adipocyte differentiation. Thus, our data demonstrate the feasibility of adipocyte-selective temporally controlled gene engineering and reveal a central role of RXRalpha in adipogenesis, probably as a heterodimeric partner for peroxisome proliferator-activated receptor gamma.

MeSH Terms (29)

Adipocytes Adipose Tissue Animals Body Temperature Body Weight Cell Differentiation Dimerization Enzyme Induction Fasting Histocytochemistry Hypothermia Integrases Lipolysis Mice Mice, Transgenic Mutagenesis Obesity Receptors, Cytoplasmic and Nuclear Receptors, Estrogen Receptors, Retinoic Acid Recombinant Fusion Proteins Retinoid X Receptors RNA, Messenger Sodium Glutamate Stem Cells Tamoxifen Time Factors Transcription Factors Viral Proteins

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