Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.

Maher N, Dillon HK, Vermund SH, Unnasch TR
Appl Environ Microbiol. 2001 67 (1): 449-52

PMID: 11133478 · PMCID: PMC92598 · DOI:10.1128/AEM.67.1.449-452.2001

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.

MeSH Terms (9)

Air Microbiology DNA, Fungal Environmental Monitoring Filtration Magnetics Pneumocystis Polycarboxylate Cement Polymerase Chain Reaction Sensitivity and Specificity

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