Qa-2-dependent selection of CD8alpha/alpha T cell receptor alpha/beta(+) cells in murine intestinal intraepithelial lymphocytes.

Das G, Gould DS, Augustine MM, Fragoso G, Sciutto E, Stroynowski I, Van Kaer L, Schust DJ, Ploegh H, Janeway CA, Scitto E
J Exp Med. 2000 192 (10): 1521-8

PMID: 11085754 · PMCID: PMC2193183 · DOI:10.1084/jem.192.10.1521

Murine intestinal intraepithelial lymphocytes (iIELs) are made up of a heterogeneous mix of T cells with unique phenotypes. Whereas CD8(+) T cells in peripheral lymphoid organs use CD8alpha/beta and are selected on MHC class Ia molecules, a majority of iIELs use CD8alpha/alpha. Here, we report that the presence of CD8alpha/alpha TCR-alpha/beta cells in iIELs is independent of classical MHC class I molecules K(b) and D(b), as illustrated by their presence in K(b)/D(b) double-knockout mice and in mice lacking a nonclassical MHC class I molecule, CD1d. Most strikingly, their presence is decreased by approximately 70% in mice lacking transporter associated with antigen processing (TAP). The TAP-dependent nonclassical MHC class I molecule Qa-2 is strongly implicated in the presence of these cells, as inferred from the low numbers of CD8alpha/alpha TCR-alpha/beta T cells in mice deficient in Qa-2 genes. Second, a Qa-2-transgenic mouse made in a Qa-2(-) strain showed an increase in the numbers of CD8alpha/alpha cells among its iIELs. Thus, the presence of CD8alpha/alpha TCR-alpha/beta cells in iIELs is mainly dependent on the nonclassical MHC class I molecule Qa-2.

MeSH Terms (13)

Animals Antigen Presentation beta 2-Microglobulin CD8-Positive T-Lymphocytes H-2 Antigens Histocompatibility Antigens Class I Intestinal Mucosa Mice Mice, Inbred Strains Mice, Transgenic Receptors, Antigen, T-Cell, alpha-beta Species Specificity T-Lymphocyte Subsets

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