PSD-95 involvement in maturation of excitatory synapses.

El-Husseini AE, Schnell E, Chetkovich DM, Nicoll RA, Bredt DS
Science. 2000 290 (5495): 1364-8

PMID: 11082065

PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.

MeSH Terms (24)

Animals Cells, Cultured Dendrites Disks Large Homolog 4 Protein Excitatory Postsynaptic Potentials Hippocampus Interneurons Intracellular Signaling Peptides and Proteins Membrane Proteins Nerve Tissue Proteins Patch-Clamp Techniques Presynaptic Terminals Protein Structure, Tertiary Pyramidal Cells Rats Receptor Aggregation Receptors, AMPA Receptors, Glutamate Receptors, N-Methyl-D-Aspartate SAP90-PSD95 Associated Proteins Synapses Synaptic Transmission Synaptic Vesicles Transfection

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